Bioinformatics analysis of T- and B-combined epitopes of OMP31 protein of Brucella melitensis in Xinjiang, China

Brucellosis is one of the major zoonoses in the word that widely inflicts health problems in animal and human. In China, brucellosis is gradually increasing in recent years, especially in the xinjiang. Therefore, this study is to predict Brucella T-and B-combined epitopes of OMP31 protein by bioinformatics software. B-cell epitopes of OMP31 protein is predicted using DNAStar and IEDB software. The prediction of T-cell epitopes of OMP31 protein is performed by SYFPEITHI and ProPred MHC Class-II Binding Peptide Prediction Server. The results suggest that some position of OMP31 protein has several higher antigen indexes. The high-scoring of dominant B-cell epitopes were located at positions 25-31, 108-114, 201-207, 152-157, 166-172, 230-238, 123-127, 96-102 and 171-176. Five potential of dominant T-cell epitopes regions were revealed, located at positions 19-30, 92-106, 106-114, 137-179, 196-228. Finally, OMP31 protein has selected the three advantages of Tand B-combined epitopes, respectively are: 108-114, 152-157, 171-176. The bioinformatics research of OMP31 protein and Tand B-combined epitopes laid to the foundation of dominant epitopes vaccine.